Abi quantifiler duo manual

 · [1] Applied Biosystems (); Quantifiler human DNA quantitation kit and Quantifiler Y human male DNA quantitation kit user’s manual, Part Number Rev A. Foster City, CA. [2] M. Barbisin, R. Fang, C.E. O'Shea, L.M. Calandro, M.R. Furtado, J.G. Shewale, Developmental validation of the Quantifiler Duo DNA. Quantifiler Kits User’s Manual ix Safety Safety Alert Words Four safety alert words appear in Applied Biosystems user documentation at points in the document where you need to be aware of relevant hazards. Each alert word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular level of observation or action, as defined below: IMPORTANT! Quantifiler™ Trio DNA Quantification Kit contains all the necessary reagents for the amplification, detection and quantification of two human-specific DNA targets and a human male-specific DNA target. The reagents are designed and optimized for use with the Applied Biosystems ™ Real-Time PCR System for Human Identification, which includes.

Applied Biosystems, Lincoln Centre Drive, Foster City, CA Hollywood, CA, October Some of the data is also presented in the Quantifiler ® Duo DNA Quantification Kit User Manual. Read the full text. About. PDF. Tools. Request permission; The Quantifiler ® Duo DNA Quantification kit enables simultaneous quantification of. Samples were submitted to quantification by real time PCR on ABI (Applied Biosystems, Foster City, CA) with the Quantifiler Duo DNA Quantification Kit (Applied Biosystems, Foster City, CA), according to the manufacturer's instructions. PCR Master Mix Applied Biosystems, µL DI Water, and 2 µL test DNA. Instrument: Real Time PCR System Applied Biosystems in emulation mode (ramp speeds of 1 °C/s) Thermal cycling: 95 °C for 10 min; 40 cycles of 95 °C for 15 s, 58 °C for 30 s, 72 °C for 45 s. Data Collection: During the 72°C for 45 s step.

The Quantifiler Duo DNA Quantification Kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. The Quantifiler® Duo DNA Quantification Kit contains all the necessary reagents for the amplification, detection, and quantification of a human‐specific DNA target and a human male‐specific DNA target. The reagents are designed and optimized for use with the Applied Biosystems® Quantifiler Kits User’s Manual ix Safety Safety Alert Words Four safety alert words appear in Applied Biosystems user documentation at points in the document where you need to be aware of relevant hazards. Each alert word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular level of observation or action, as defined below: IMPORTANT!.